| Simultaneous determination of andrographolide and dehydroandrographolide in Andrographis paniculata and Chinese medicinal preparations by microemulsion electrokinetic chromatography | |
| Department | 甘肃省天然药物重点实验室 |
| Zhao YF(赵艳芳); Luo XP(罗兴平); Zhai ZD(翟宗德); Chen LR(陈立仁); Li YM(李永民) | |
| 2006 | |
| Source Publication | Journal of Pharmaceutical and Biomedical Analysis
 |
| ISSN | 0731-7085 |
| Volume | 40Pages:157-161 |
| Abstract | The present paper describes the development of a microemulsion electrokinetic chromatographic (MEEKC) method for simultaneous determination of andrographolide and dehydroandrographolide in traditional Chinese medicines and Chinese medicinal preparations. The MEEKC method involved the use of sodium dodecyl sulfate (SDS) as surfactant, heptane as organic solvent and butan-1-ol as co-solvent. The effect of temperature and pH of running buffers on separation were examined. The optimized conditions (heptane 0.81% (w/w), SDS 3.31% (w/w), butan-1-ol 6.61% (w/w) and 10mM sodium tetraborate buffer, pH 9.2) allowed a useful and good reproducible separation of the studied analytes to be achieved. |
| Keyword | Microemulsion Electrokinetic Chromatography Andrographis Paniculata Andrographolide Dehydroandrographolide |
| Subject Area | 分析化学与药物化学 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物手性分离研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1493 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Chen LR(陈立仁) |
| Recommended Citation GB/T 7714 | Zhao YF,Luo XP,Zhai ZD,et al. Simultaneous determination of andrographolide and dehydroandrographolide in Andrographis paniculata and Chinese medicinal preparations by microemulsion electrokinetic chromatography[J]. Journal of Pharmaceutical and Biomedical Analysis,2006,40:157-161. |
| APA | 赵艳芳,罗兴平,翟宗德,陈立仁,&李永民.(2006).Simultaneous determination of andrographolide and dehydroandrographolide in Andrographis paniculata and Chinese medicinal preparations by microemulsion electrokinetic chromatography.Journal of Pharmaceutical and Biomedical Analysis,40,157-161. |
| MLA | 赵艳芳,et al."Simultaneous determination of andrographolide and dehydroandrographolide in Andrographis paniculata and Chinese medicinal preparations by microemulsion electrokinetic chromatography".Journal of Pharmaceutical and Biomedical Analysis 40(2006):157-161. |
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