| Rapid separation and determination of resibufogenin and cinobufagin in toad venom and Liushen tablet by β-cyclodextrin modified micellar electrokinetic chromatography | |
| Department | 甘肃省天然药物重点实验室 |
| Zhao YF(赵艳芳); Luo XP(罗兴平); Ming YF(明永飞); Chen LR(陈立仁); Li YM(李永民) | |
| 2006 | |
| Source Publication | Journal of Pharmaceutical and Biomedical Analysis
 |
| ISSN | 0731-7085 |
| Volume | 41Pages:124-128 |
| Abstract | A rapid cyclodextrin modified micellar electrokinetic chromatography (CD-MEKC) method was proposed for the determination of resibufogenin and cinobufagin in the Chinese herbal extracts from toad venom and its medicinal preparation (Liushen tablet). The two components have the close structural similarity and similar hydrophobicity, which result in poor resolution in normal MEKC. The addition of neutral b- CD to the MEKC system was found to improve the separation of the studied compounds. The effects of several CD-MEKC parameters on the resolutions were evaluated systematically. Based on the investigation, a background electrolyte solution consisting of 10mM borate buffer adjusted to pH 8.5, 40mM sodium dodecyl sulfate (SDS), 12mM -CD and 10% (v/v) of methanol was found to be optimal conditions for the fast separation. The contents of resibufogenin and cinobufagin were successfully determined within 5 min, with satisfactory repeatability and recovery. |
| Keyword | Micellar Electrokinetic Chromatography B-cyclodextrin Resibufogenin Cinobufagin Toad Venom |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the Foundation for “Bairen Jihua” of CAS in 2001; National Natural Science Foundation of China (no. 20375045) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物手性分离研究组 |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1459 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Chen LR(陈立仁) |
| Recommended Citation GB/T 7714 | Zhao YF,Luo XP,Ming YF,et al. Rapid separation and determination of resibufogenin and cinobufagin in toad venom and Liushen tablet by β-cyclodextrin modified micellar electrokinetic chromatography[J]. Journal of Pharmaceutical and Biomedical Analysis,2006,41:124-128. |
| APA | 赵艳芳,罗兴平,明永飞,陈立仁,&李永民.(2006).Rapid separation and determination of resibufogenin and cinobufagin in toad venom and Liushen tablet by β-cyclodextrin modified micellar electrokinetic chromatography.Journal of Pharmaceutical and Biomedical Analysis,41,124-128. |
| MLA | 赵艳芳,et al."Rapid separation and determination of resibufogenin and cinobufagin in toad venom and Liushen tablet by β-cyclodextrin modified micellar electrokinetic chromatography".Journal of Pharmaceutical and Biomedical Analysis 41(2006):124-128. |
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