| Separation and determination of aristolochic acids in herbal medicines by microemulsion electrokinetic chromatography | |
| Department | 甘肃省天然药物重点实验室 |
Zhai ZD(翟宗德); Luo XP(罗兴平); Shi YP(师彦平)
| |
| 2006 | |
| Source Publication | Analytica Chimica Acta
 |
| ISSN | 0003-2670 |
| Volume | 561Pages:119-125 |
| Abstract | A microemulsion electrokinetic chromatography (MEEKC) method has been firstly developed and validated for the separation of aristolochic acids A, B, C and D, 7-hydroxy-aristolochic acid A and aristolic acid in herbal medicines. With the optimized experimental conditions (octane 0.81% (w/w), SDS 3.31% (w/w), butan-1-ol 6.61% (w/w) and 10mM sodium tetraborate buffer 89.27% (w/w), with 15 kV as applied voltage, pH 9.2, 25℃ as capillary temperature), all of the six analytes were baseline-separated (any resolution was over 2) in peaks of good shapes within 16 min. Aristolochic acids A, B, C and aristolic acid were quantitative determined and acceptable precision (intra-day repeatability (n = 6) and inter-day reproducibility (n = 3) of the method in terms of migration times were within 0.98 and 2.75% R.S.D. in terms of migration times and within 5.09 and 5.93% R.S.D. in terms of peak areas, respectively) and linearity (r > 0.995) and recovery (ranged between 97 and 104%) were obtained. Moreover, the method was successfully validated and applied to the determination of four analytes in herbal medicines. |
| Keyword | Microemulsion Electrokinetic Chromatography Aristolochic Acids Chinese Herbs Nephropathy |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the “Bairen Jihua” Foundation of Chinese Academy of Sciences (CAS) in 2000;National Natural Science Foundation of China (NSFC No. 20475057) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1447 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Shi YP(师彦平) |
| Recommended Citation GB/T 7714 | Zhai ZD,Luo XP,Shi YP. Separation and determination of aristolochic acids in herbal medicines by microemulsion electrokinetic chromatography[J]. Analytica Chimica Acta,2006,561:119-125. |
| APA | 翟宗德,罗兴平,&师彦平.(2006).Separation and determination of aristolochic acids in herbal medicines by microemulsion electrokinetic chromatography.Analytica Chimica Acta,561,119-125. |
| MLA | 翟宗德,et al."Separation and determination of aristolochic acids in herbal medicines by microemulsion electrokinetic chromatography".Analytica Chimica Acta 561(2006):119-125. |
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| Separation and deter（245KB） | 开放获取 | License | View Application Full Text | |||
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