| Separation and determination of flavonoids in Ixeridium gracile by capillary electrophoresis | |
| Department | 甘肃省天然药物重点实验室 |
Zhang Y(张玉); Zhao L(赵亮) ; Shi YP(师彦平)
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| 2007 | |
| Source Publication | J Chromatogr Sci
 |
| ISSN | 0021-9665 |
| Volume | 45Pages:600-604 |
| Abstract | A simple and rapid capillary electrophoresis method has been developed for the quantitative analysis of three active compounds: (3R)-7,2'-dihydroxy-3',4'-dimethoxy-isoflavan, kaempferol, and quercetin in Ixeridium gracile. The buffer solution used in this method is 20 mmol/L borate at pH 9.5. The effects of pH value, borate concentration, and applied voltage on migration behavior are systematically investigated. Regression equations reveal good linear relationships (correlation coefficients: 0.9986, 0.9997, and 0.9999) between the peak area of each compound and its concentration. The relative standard deviations of the migration time and peak area are less 1.12% and 3.11% (intraday), and 1.43% and 3.52% (interday), respectively, under the optimized separation conditions. The contents of the three flavonoids in I. gracile are successfully determined within 7.8 min, with satisfactory repeatability and recovery. |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | National Natural Science Foundation of China (No. 20475057);the Natural Science Foundation of Gansu Province (No. 3ZS051-A25-077) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 ; 药物手性分离研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1416 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Shi YP(师彦平) |
| Recommended Citation GB/T 7714 | Zhang Y,Zhao L,Shi YP. Separation and determination of flavonoids in Ixeridium gracile by capillary electrophoresis[J]. J Chromatogr Sci,2007,45:600-604. |
| APA | 张玉,赵亮,&师彦平.(2007).Separation and determination of flavonoids in Ixeridium gracile by capillary electrophoresis.J Chromatogr Sci,45,600-604. |
| MLA | 张玉,et al."Separation and determination of flavonoids in Ixeridium gracile by capillary electrophoresis".J Chromatogr Sci 45(2007):600-604. |
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| Separation and deter（125KB） | 开放获取 | License | View Application Full Text | |||
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