| Separation and determination of flavonoids in Lamiophlomis rotata by capillary electrophoresis using borate as electrolyte | |
| Department | 甘肃省天然药物重点实验室 |
Luo MN(罗米娜); Lu HW(卢华卫); Ma HQ(马华侨); Zhao L(赵亮) ; Liu X(刘霞); Jiang SX(蒋生祥)
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| 2007 | |
| Source Publication | Journal of Pharmaceutical and Biomedical Analysis
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| ISSN | 0731-7085 |
| Volume | 44Pages:881–886 |
| Abstract | A capillary electrophoresis methodwas developed for the simultaneous determination of five flavonoids such as luteolin-7-O-glucoside, isorhamnetin, apigenin, luteolin and quercetin in Lamiophlomis rotata (Benth.) Kudo. Optimal conditions were obtained at pH 9.0 with 30mM borate as buffer containing 8% (v/v) acetonitrile, 20 kV as driving voltage and 210 nm as detection wavelength. The association constant K and the change in Gibbs free energy ( G) of the interaction of flavonoids with borate anion ion (a typical ion-dipole or ion-induced dipole interaction) were calculated for the quantitative evaluation and characterization of the interaction. The described method was successfully applied for the rapid and efficient quality control by quantifying flavonoids in L. rotata. Repeatability tests showed that the R.S.D.s of both intra- and inter-day migration times and peak areas were less than 5%. The LOD of the five flavonoids was less than 3.75 mg/l. Recovery results ranged from 94.2% to 105.1%. |
| Keyword | Flavonoid Capillary Electrophoresis Lamiophlomis Rotata Association Constant |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the Major Frontier Projects of the Chinese Academy of Sciences (No. KGCX2-213-08);the West Light Program of China (2003);the Young-Aged Scientific Foundation of Gansu Province (No. 3ZS041-A25-23) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物分离材料研究组 ; 药物手性分离研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1410 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Jiang SX(蒋生祥) |
| Recommended Citation GB/T 7714 | Luo MN,Lu HW,Ma HQ,et al. Separation and determination of flavonoids in Lamiophlomis rotata by capillary electrophoresis using borate as electrolyte[J]. Journal of Pharmaceutical and Biomedical Analysis,2007,44:881–886. |
| APA | 罗米娜,卢华卫,马华侨,赵亮,刘霞,&蒋生祥.(2007).Separation and determination of flavonoids in Lamiophlomis rotata by capillary electrophoresis using borate as electrolyte.Journal of Pharmaceutical and Biomedical Analysis,44,881–886. |
| MLA | 罗米娜,et al."Separation and determination of flavonoids in Lamiophlomis rotata by capillary electrophoresis using borate as electrolyte".Journal of Pharmaceutical and Biomedical Analysis 44(2007):881–886. |
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