| Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs | |
| Department | 甘肃省天然药物重点实验室 |
Tang H(汤浩); Min GT(闵光涛); Ge J(葛晋); Li YM(李玉民); Liu X(刘霞) ; Jiang SX(蒋生祥)
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| 2008 | |
| Source Publication | Journal of Ethnopharmacology
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| ISSN | 0378-8741 |
| Volume | 117Issue:3Pages:420-426 |
| Abstract | Aim of the study: Chi-Zhi-Huang decoction (PGR) is one of the traditional Chinese medicine (TCM) preparations with unique effect on withdrawing jaundice and has been used to treat icteric patients in China for many years. In this research, we aim at to evaluate the potential activity of PGR in restoring hepatic drug metabolism in a damaged liver. Materials and methods: A cocktail approach with caffeine (10 mg/kg), dapsone (10 mg/kg) and chlorzoxazone (20 mg/kg) respectively as probe drug of cytochrome P450 (CYP) isoform of CYP 1A2, 3A4 and 2E1 was used to evaluate its possible effects on Phase I oxidative metabolism. Pretreated with three dosages of PGR water extract (0.75, 1.5 and 3 g/kg, po) for 5 days, male Wistar rats (220–240 g) were intoxicated by phenylisothiocyanate (PITC, 100 mg/kg, po) 24 h before probes intravenous injection. The pharmacokinetics of the probes in the blood was determined simultaneously by HPLC, and their non-compartmental parameterswere used to evaluate the metabolic difference among the groups. Moreover, the levels of liver enzymes (ALT, AST, ALP) and bilirubins were also measured for insight of liver function. Results: The findings in this study suggest that PGR induces CYP 3A4, does not have much effect on CYP 2E1, and inhibits CYP 1A2 at high dosage.Conclusion: The current pharmacokinetic approach allowed the protective effects of PGR on oxidative drug metabolism in damaged liver to be systemically examined and will certainly help in the explanation of synergistic effect of the composites formula. |
| Keyword | Chi-zhi-huang Decoction Phase i Drug Metabolism Cocktail Probe Drugs High Performance Liquid Chromatography |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the Natural Science Foundation of Gansu Province, China (No. 3YS051-A25-022). |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物分离材料研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1387 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Jiang SX(蒋生祥) |
| Recommended Citation GB/T 7714 | Tang H,Min GT,Ge J,et al. Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs[J]. Journal of Ethnopharmacology,2008,117(3):420-426. |
| APA | 汤浩,闵光涛,葛晋,李玉民,刘霞,&蒋生祥.(2008).Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs.Journal of Ethnopharmacology,117(3),420-426. |
| MLA | 汤浩,et al."Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs".Journal of Ethnopharmacology 117.3(2008):420-426. |
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