| Preparative isolation and purification of steviol glycosides from Stevia rebaudiana Bertoni using high-speed counter-current chromatography | |
| Department | 甘肃省天然药物重点实验室 |
Huang XY(黄新异) ; Fu JF(伏军芳); Di DL(邸多隆)
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| 2010 | |
| Source Publication | Separation and purification technology
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| ISSN | 1383-5866 |
| Volume | 71Pages:220-224 |
| Abstract | Three steviol glycosides, stevioside, rebaudioside A and rebaudioside C were successfully isolated and purified from the extract of leaves of Stevia rebaudiana Bertoni by high-speed counter-current chromatography (HSCCC). Based on the principle of the partition coefficient values (k) for target compounds and the separation factor (˛) between targets compounds, the two-phase solvent system which contains n-hexane–n-butanol–water at an optimized volume ratio of 1.5:3.5:5 (v/v/v) was selected for the HSCCC separation and the lower phase was employed as the mobile phase in the head to tail elution mode. In a single operation, 200mg of the crude extract yielded pure stevioside (54 mg), rebaudioside A (36 mg), and rebaudioside C (13 mg) with the purities of 98.3%, 98.5% and 97.6%, respectively. The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the structures of the three steviol glycosides were identified by 1H NMR, 13C NMR and DEPT-NMR. |
| Keyword | High-speed Counter-current Chromatography Steviol Glycosides Stevioside Rebaudioside a Rebaudioside c |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the Natural Sciences Foundation of China (20775083);the “Hundred Talents Program” of the Chinese Academy of Sciences (CAS) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物工艺标准研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1226 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Di DL(邸多隆) |
| Recommended Citation GB/T 7714 | Huang XY,Fu JF,Di DL. Preparative isolation and purification of steviol glycosides from Stevia rebaudiana Bertoni using high-speed counter-current chromatography[J]. Separation and purification technology,2010,71:220-224. |
| APA | 黄新异,伏军芳,&邸多隆.(2010).Preparative isolation and purification of steviol glycosides from Stevia rebaudiana Bertoni using high-speed counter-current chromatography.Separation and purification technology,71,220-224. |
| MLA | 黄新异,et al."Preparative isolation and purification of steviol glycosides from Stevia rebaudiana Bertoni using high-speed counter-current chromatography".Separation and purification technology 71(2010):220-224. |
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