| RP-HPLC and NMR Study of Antioxidant Flavonoids in Extract from Gentiana piasezkii | |
| Department | 甘肃省天然药物重点实验室 |
Wu QX(武全香); Chen J(陈娟) ; Shi YP(师彦平)
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| 2010 | |
| Source Publication | J. Anal. Chem.
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| ISSN | 1061-9348 |
| Volume | 65Issue:3Pages:298-304 |
| Abstract | A reversed_phase high performance liquid chromatography (RP_HPLC) separation with photo_diode array detection (PDA) was developed for the simultaneous determination of five flavonoids, lutonarin(LO), saponarin (SA), isoorient (IO), 7_O_feruloylorientin (FO) and luteolin (LE) in the extract of Gentiana piasezkii, a popular forage grass, pharmaceutical and ornamental plant in China. The optimized method was achieved for the separation and detection of selected constituents, using acetonitrile_1% acetic acid as the mobile phase with gradient elution at a flow rate of 0.8 mL/min and 350 nm as the detection wavelength. The contents of the five flavonoids, i.e., LO, SA, IO, FO and LE in the plant of G. piasezkii were 0.1524, 0.0673, 0.300, 0.0552 and 0.0052%, respectively. The compounds possess similar structures and have been studied by nuclear magnetic resonance (NMR) spectroscopy. |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the National Basic Research Program (program 973) of China (2007CB108903);the National Natural Science Foundation of China (no. 20621091) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1201 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Shi YP(师彦平) |
| Recommended Citation GB/T 7714 | Wu QX,Chen J,Shi YP. RP-HPLC and NMR Study of Antioxidant Flavonoids in Extract from Gentiana piasezkii[J]. J. Anal. Chem.,2010,65(3):298-304. |
| APA | 武全香,陈娟,&师彦平.(2010).RP-HPLC and NMR Study of Antioxidant Flavonoids in Extract from Gentiana piasezkii.J. Anal. Chem.,65(3),298-304. |
| MLA | 武全香,et al."RP-HPLC and NMR Study of Antioxidant Flavonoids in Extract from Gentiana piasezkii".J. Anal. Chem. 65.3(2010):298-304. |
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