| Determination of aristolochic acid in urine using hollow fiber liquid-phase microextraction combined with high performance liquid chromatography | |
| Department | 甘肃省天然药物重点实验室 |
Yang Y(杨阳); Chen J(陈娟) ; Shi YP(师彦平)
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| 2010 | |
| Source Publication | Biomed. Chromatogr.
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| ISSN | 0269-3879 |
| Volume | 24Pages:1350-1355 |
| Abstract | A simple and efficient hollow fiber liquid-phase microextraction (HF-LPME) technique in conjunction with highperformance liquid chromatography is presented for extraction and quantitative determination of aristolochic acid I in human urine samples. Several parameters influencing the efficiency of HF-LPME were investigated and optimized, including extraction solvent, stirring rate, extraction time, pH of donor phase and acceptor phase. Excellent sample clean-upwas observed and good linearity with coefficient of 0.9999 was obtained in the range of 15.4–960 mg/L. This method provided a 230-fold enrichment factor and good repeatabilitywith relative standard deviations (RSD) lower than 6.0%.The limit of detection value for the analyte in urine samplewas 0.01 mg/L at a signal-to-noise ratio of 3.The extraction recovery fromurine sampleswas 61.8% with an RSD of 9.71%. |
| Keyword | Hollow Fiber Liquid-phase Microextraction High-performance Liquid Chromatography Aristolochic Acid Urine Sample |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | National Key Technology Research and Development Program of China (no. 2007BAI37B05);West Light Program of Chinese Academy of Sciences (2007) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1199 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Chen J(陈娟); Shi YP(师彦平) |
| Recommended Citation GB/T 7714 | Yang Y,Chen J,Shi YP. Determination of aristolochic acid in urine using hollow fiber liquid-phase microextraction combined with high performance liquid chromatography[J]. Biomed. Chromatogr.,2010,24:1350-1355. |
| APA | 杨阳,陈娟,&师彦平.(2010).Determination of aristolochic acid in urine using hollow fiber liquid-phase microextraction combined with high performance liquid chromatography.Biomed. Chromatogr.,24,1350-1355. |
| MLA | 杨阳,et al."Determination of aristolochic acid in urine using hollow fiber liquid-phase microextraction combined with high performance liquid chromatography".Biomed. Chromatogr. 24(2010):1350-1355. |
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